Signal peptide of potato PinII enhances the expression of Cry1Ac in transgenic tobacco.

Acta biochimica et biophysica Sinica

PubMedID: 15295648

Liu YJ, Yuan Y, Zheng J, Tao YZ, Dong ZG, Wang JH, Wang GY. Signal peptide of potato PinII enhances the expression of Cry1Ac in transgenic tobacco. Acta Biochim Biophys Sin (Shanghai). 2004;36(8):553-8.
The modified Cry1Ac was expressed in transgenic tobacco plants. To allow secretion of the Cry1Ac protein into the intercellular space, the signal peptide sequence of potato proteinase inhibitor II (pinII) was N-terminally fused to the Cry1Ac encoding region. Expression of Cry1Ac in transgenic tobacco plants was assayed with ELISA. The results showed that pinII signal peptide sequence enhanced the expression of Cry1Ac protein and led to the secretion of the Cry1Ac protein in transgenic tobacco plants. GFP gene was also fused to the signal peptide sequence and transformed to tobacco. The results of fluorescent detection showed that GFP had localized in the apoplast of transgenic plants.