Application of recombinant fluorescent mammalian cells as a toxicity biosensor.

Water science and technology : a journal of the International Association on Water Pollution Research

PubMedID: 12227603

Kim EJ, Lee Y, Lee JE, Gu MB. Application of recombinant fluorescent mammalian cells as a toxicity biosensor. Water Sci Technol. 2002;46(3):51-6.
With respect to developing a more sensitive biosensor, a recombinant fluorescent Chinese Hamster Ovary cell line was used for the monitoring of various toxicants. Both cell lines, EFC-500 and KFC-A10, were able to detect toxicants sensitively. They were characterized with mitomycin C and gamma-ray as genotoxicants and bisphenol A, nonylphenol, ziram and methyl bromide as possible and known EDCs. When compared to each other, the response of KFC-A10 was generally more informative and sensitive. Compared to typical bacterial biosensor systems, these cell lines offered a sensitivity of 2- to 50-fold greater for the tested chemicals. Based on these results, the use of mammalian cells offers a sensitive biosensor system that is not only fast, cheap and reproducible but also capable of monitoring the endocrine-like characteristics of environmental toxicants.