[Optimization of parameters of exogene transfection of bovine fetal fibroblasts in vitro mediated by liposome].

Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji

PubMedID: 15979962

Li Y, Wu KF, Guo XD, Guo JT, Bou SG. [Optimization of parameters of exogene transfection of bovine fetal fibroblasts in vitro mediated by liposome]. Yi Chuan. 2002;24(6):653-5.
pEGFP-C1 eucaryon expression vector was successfully transfected by liposome into bovine fetal fibroblasts. We investigated the effect of parameter such as the dose of DNA and liposome,number of cell transfected and exposure time of the cell to the DNA-liposome complexes. It was indicated that GFP (green fluorescent protein) expression was enhanced as the dose of DNA and liposome increased and on decline as the exposure time was prolonged. The improvement of transfection efficiency depent on the suitable cell number.