Photodynamic action of actinomycin D: an EPR spin trapping study.

Biochimica et biophysica acta

PubMedID: 11420136

Pan JX, Liu Y, Zhang SP, Tu TC, Yao SD, Lin NY. Photodynamic action of actinomycin D: an EPR spin trapping study. Biochim Biophys Acta. 2001;1527(1-2):1-3.
Actinomycin D is one of the most widely studied anticancer antibiotic that binds to both double-stranded and single-stranded DNA, and this binding greatly enhances the DNA photosensitization. By use of electron paramagnetic resonance spin trapping techniques, both superoxide radical anion and the radical anion of actinomycin D were identified as important intermediates in the photodynamic process. A mechanism of electron transfer from a DNA base to excited actinomycin D was proposed. These novel findings may shed new light on future application of this drug in photodynamic therapy or cleavage of DNA in unique and controllable ways.